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The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
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Overexpression of the gene has actually been noticed in lots of tumors, which ends up in chromosome instability, and thus may perhaps contribute to tumorigenesis. This protein was uncovered to affiliate with, and become associated with, the phosphorylation of NPAT protein (nuclear protein mapped towards the ATM locus), which participates in mobile-cycle regulated histone gene expression and performs a essential part in endorsing mobile-cycle progression during the absence of pRB. Two alternatively spliced transcript variants of this gene, which encode unique isoforms, are already explained. Two added splice variants had been claimed but thorough nucleotide sequence info isn't nonetheless available.
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Blocking peptides are peptides that bind especially into the target antibody and block antibody binding. These peptide normally contains the epitope acknowledged because of the antibody.
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This guideline outlines even further attainable optimization of cellular and immunoassay parameters to ensure the very best benefits are...
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This manual outlines even more attainable optimization of mobile and immunoassay parameters to guarantee the very best effects are...
Antibodies sure to the blocking peptide no more bind on the epitope to the focus on protein. By comparing the staining from your blocked antibody compared to the antibody alone, you can see which staining is unique.
Concentrate on Facts Cyclin E belongs to your hugely conserved cyclin loved ones, whose associates are characterized by a extraordinary periodicity in protein abundance from the cell cycle. Cyclins function as regulators of CDK kinases. Distinctive cyclins show distinct expression and degradation styles which add for the temporal coordination of each mitotic event. Cyclin E kinds a complex with and capabilities being a regulatory subunit of CDK2, whose activity is necessary for cell cycle G1/S changeover. This protein accumulates with the G1-S phase boundary and is particularly degraded as cells progress through S period.
The measurement of protein phosphorylation is a great tool for measuring the modulation of receptor activation by both antibodies...
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